MOLECULAR SELF-ASSEMBLING OF BIOCATALYTIC NANOCONSTRACTIONS WITH ELECTROCHEMICAL AND PHOTOCHEMICAL REGENERATION OF NICOTINAMIDE COENZYMES

P. Gladyshev¹, D. Gladeshev², A. Rybakova³

¹International University of Nature, Society and Man "Dubna"; 19, Universitetskaya St., Dubna, Moscow Region, 141980, Russia; glad@uni-dubna.ru
² Innovative Alliance Ltd.; 5, Zhukovskogo St., Dubna, Moscow Region, 141980, Russia; furman@dubna.ru
³ Applied Acoustics Research Institute; 7A, 9th May Str., Dubna, Moscow Region, 141980, Russia; nasty.rybakova@niipa.ru

The problems of nanostructuring of immobilized enzyme-coenzyme systems with electrochemical and photochemical cofactor regeneration are discussed here. Such nanoconstructions were defined as reconstructed enzyme systems, particularly as reconstructed oxydoreductases systems. The main requirement for such nanostructions is the bounding of all structural blocks in one construction, which provide target biocatalysis and electrochemical or photochemical cofactor regeneration processes. Furthermore, different mechanisms of electron transfer from an active center of oxydoreductases to an electrode matrix were investigated, as well as optimal constructions for immobilized enzyme-coenzyme systems.

Key words: immobilized enzyme-coenzyme systems, oxydoreductases, HAD, HADF, electrochemical and photochemical cofactor regeneration.

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